GlowCas9: The Revolutionary Gene Editing Tool with Real-time Visualization

Scientists from the Bose Institute in India have developed an innovative variant of the CRISPR-Cas9 known as GlowCas9, which emits light during the gene editing process. This allows researchers to observe gene editing in real-time in living cells, enhancing the accuracy and safety of gene therapy. By using a split nano-luciferase enzyme derived from deep-sea shrimp proteins, GlowCas9 produces bioluminescence, enabling precise monitoring without disrupting cell integrity. This advancement, published in Angewandte Chemie International Edition, opens new pathways for gene editing in both human health and plant biotechnology.

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